Fig. 4.
Fig. 4. Comparative LacZ staining of representative sections from Flt-1-lacZ-Hprt and VWF-lacZ-Hprt mice. / LacZ staining of 10 μm tissue sections was performed in parallel. Left column indicates Flt-1-lacZ-Hprt mice. Right column indicates VWF-lacZ-Hprt mice. In Flt-1-lacZ-Hprt hearts, β-galactosidase activity was present in the endothelium and smooth muscle cells of occasional large arteries as well as in cardiomyocytes (A). In VWF-lacZ-Hprt hearts, LacZ expression was detected in the endothelial lining of a subset of capillaries (B,H). Flt-1-lacZ-Hprt mice revealed reporter gene activity in the glomeruli and small arterioles of the kidney (C,G), and in microvessels of the spleen (E). In contrast, the X-Gal reaction product was undetectable in the kidney (D) and spleen (F) of VWF-lacZ-Hprt mice. A-F, × 100 optical magnification; G and H, × 1000 optical magnification.

Comparative LacZ staining of representative sections from Flt-1-lacZ-Hprt and VWF-lacZ-Hprt mice.

LacZ staining of 10 μm tissue sections was performed in parallel. Left column indicates Flt-1-lacZ-Hprt mice. Right column indicates VWF-lacZ-Hprt mice. In Flt-1-lacZ-Hprt hearts, β-galactosidase activity was present in the endothelium and smooth muscle cells of occasional large arteries as well as in cardiomyocytes (A). In VWF-lacZ-Hprt hearts, LacZ expression was detected in the endothelial lining of a subset of capillaries (B,H). Flt-1-lacZ-Hprt mice revealed reporter gene activity in the glomeruli and small arterioles of the kidney (C,G), and in microvessels of the spleen (E). In contrast, the X-Gal reaction product was undetectable in the kidney (D) and spleen (F) of VWF-lacZ-Hprt mice. A-F, × 100 optical magnification; G and H, × 1000 optical magnification.

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