Direct sequence analyses of PCR-amplified PIG-A DNA in CD59-deficient CD34⁺ cells and granulocytes and in CD59⁺granulocytes from PNH patients.

Direct sequence analyses of PCR-amplified PIG-A DNA from bone marrow CD34⁺ cells or peripheral blood granulocytes from 3 PNH patients and a healthy volunteer were performed as described in “Patients, materials, and methods.” The results, with controls for each mutation shown in the right column, were obtained by analyses of PIG-A DNA in CD59⁺ populations of peripheral blood granulocytes from the PNH patients. Normal and mutated alleles are presented above each graph in the upper and lower rows, respectively.