T/B lymphocyte peripheral reconstitution after gene transfer.

Peripheral blood was tested for T- and B-cell counts throughout the experiment. Nucleated blood cells were counted and stained for immunocytofluorometry (black: B220⁺IgM⁺; dotted: CD3⁺TCRαβ⁺; white: CD3⁺CD4⁺; gray: CD3⁺CD8⁺). (A) Number of nucleated cells in peripheral blood of RAG-2–transduced mice (n = 11). (B) Number of nucleated cells in peripheral blood of mock-transduced mice (n = 7). (C) Number of nucleated cells in peripheral blood of C57BL/6 mice (n = 4). RAG2−/− mice consistently lacked any expression of B220⁺IgM⁺, CD3⁺TCRαβ⁺, CD3⁺CD4⁺, or CD3⁺CD8⁺cells throughout their lives. Data are expressed as means ± SEM.