Methylated α^π-globinpromoter interacts with MeCPC.

(A) A ³²P-labeled mock-methylated or methylatedα^π-globin probe was incubated with increasing amounts of primary erythroid cell nuclear extract, followed by agarose gel electrophoresis and autoradiography. (B) EMSA analysis of MeCPC competition in erythroid cell nuclear extract. A³²P-labeled methylated α^π-globinprobe was incubated with nuclear extract from 11-day avian erythroid cells with increasing molar excess of mock-methylated or methylatedα^π-globin fragment, as indicated above each lane, followed by agarose gel electrophoresis and autoradiography.