Immunophenotypic and morphologic analysis.

(A) Immunophenotypic profile of GFP⁺, CD34⁺, CD13⁻ cells expressing N-Ras* compared with corresponding controls; day-8 cultures were triple-labeled with antibodies to CD34, CD13, and to one of the determinates indicated. Error bars represent SD (n = 4); *P < .05, **P < .01. (B) Morphology of day-8 cultures and (C) morphology of CD34⁺cells from day-8 cultures. In each case, cytospin preparations were stained with Wright-Giemsa (original magnification × 400). Note: Vacuolization is a common feature of cultured human erythroblasts.