Fig. 5.
Effect of N-Ras* on the distribution of PKC activity.
(A) Total PKC activity (106 cell equivalents) in cytosolic [C], membrane [M], and nuclear [N] fractions (day-7 cells). Error bars represent SD (n = 5); *P < .05. (B) Corresponding total PKC expression by Western blot: relative intensity of expression in N-Ras* versus control cells was 2.3-fold ± 0.28-fold (n = 3); P < .05. (C) Phospho-PKC expression by Western blot: Relative intensity of expression in N-Ras* versus control cells was 4.0-fold ± 1.4-fold.