Basal and GATA-1–induced transactivation of the fur P1, P1A, and P1B promoters.

(A) Schematic representation of localization and sequence of the GATA-1 recognition motifs present in the 5′ noncoding exons 1, 1A, and 1B of the human fur gene. (B) Transient cotransfection of Dami cells, with fur P1, P1A, or P1B promoters and either pMGS control vector or pMGS–GATA-1 vector. Before transfection, Dami cells were differentiated overnight with 100 nM PMA. Luciferase activity is expressed as fold-increase relative to P1 promoter activity (set at 1) cotransfected with pMGS control vector. Data are expressed as the mean ± SEM; n = 3. **P < .001, compared with promoter constructs without GATA-1.