Transactivation of the fur P1 promoter by GATA-1 and FOG-1.

(A) Dami cells were differentiated overnight with 100 nM PMA, or (B) HepG2 cells were transiently cotransfected with fur P1 promoter and either pMGS control vector or pMGS-GATA-1, in combination with pCDNA3 control vector or pCDNA3–FOG-1. Luciferase activity is expressed as fold-increase relative to the activity of P1 promoter cotransfected with pMGS control vector (value set at 1). Data are expressed as the mean ± SEM; n = 3. **P < .001, compared with P1 promoter activity.