Challenge of fully reconstituted BM chimeric mice with an injection of wild-type BM cells.

(A-B) Chimeric mice were generated in the Ly-5.1 background and were then challenged with a dose of wild-type HW80 BM cells. Following injection, the mice were bled serially and analyzed for the presence of Hb_d red blood cells by hemoglobin electrophoresis with analyses continued for up to 18 weeks. At the time of killing, BM cells from some mice were transplanted into lethally irradiated Hb_s secondary recipients (panel A, bottom). (C-D) Chimeric mice were generated in the HW80 background and then challenged with a dose of wild-type Ly-5.1 BM cells. Following injection, the mice were bled serially and analyzed for the presence of Ly-5.1–positive cells by flow cytometry for up to 16 weeks following challenge.