American Society of Hematology

Fig. 2.

$Fig. 2. The role of CD52+ cells in the allogeneic mixed lymphocyte reaction. / (A) The effect of depletion of CD52-expressing cells from the stimulatory arm of the allo-MLR. The proliferation of purified allogeneic T lymphocytes in response to unfractionated or CD52-depleted, irradiated allogeneic PBMCs is shown for 3 separate stimulator/responder pairs and expressed as the mean of triplicate readings + SEM. (B) The effect of lineage depletion, CD52 depletion, CD52 and CD123 depletion, or add-back of purified CD3+, CD14+, and CD19+ cells to the CD52-depleted fraction on the allo-MLR. Lineage depletion maintained or enhanced the allo-MLR. Add-back of monocytes and lymphocytes variably reconstituted the allogeneic response to CD52-depleted cells, although not to the level observed in the lineage− cells. The CD52−CD123−fraction elicited a response similar to or further reduced compared to that of CD52− cells. The significance of the results obtained is shown above the columns in question and the values for nonadjacent columns are indicated by double arrows.$

The role of CD52⁺ cells in the allogeneic mixed lymphocyte reaction.

(A) The effect of depletion of CD52-expressing cells from the stimulatory arm of the allo-MLR. The proliferation of purified allogeneic T lymphocytes in response to unfractionated or CD52-depleted, irradiated allogeneic PBMCs is shown for 3 separate stimulator/responder pairs and expressed as the mean of triplicate readings + SEM. (B) The effect of lineage depletion, CD52 depletion, CD52 and CD123 depletion, or add-back of purified CD3⁺, CD14⁺, and CD19⁺ cells to the CD52-depleted fraction on the allo-MLR. Lineage depletion maintained or enhanced the allo-MLR. Add-back of monocytes and lymphocytes variably reconstituted the allogeneic response to CD52-depleted cells, although not to the level observed in the lineage⁻ cells. The CD52⁻CD123⁻fraction elicited a response similar to or further reduced compared to that of CD52⁻ cells. The significance of the results obtained is shown above the columns in question and the values for nonadjacent columns are indicated by double arrows.

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