Fig. 6.
Analysis of specific degradation of HS and MPD by gel filtration chromatography.
3H-labeled HS (A, C) and MPD (B, D) were degraded by exhaustive treatment with low pH nitrous acid (A, B) and heparinase III (C, D), as described in “Materials and methods.” Digests were analyzed by chromatography on a Biogel P10 column. Distinct oligosaccharide peaks are labeled according to the number of monosaccharide units.