Inappropriate expression of hepcidin in adenoma tissue from GSD patients.

(A) Hepcidin is abundantly expressed in liver tissue from a healthy adult male (lane 1) but significantly decreased in the unaffected liver tissue from patient A (lane 2). In contrast, hepcidin mRNA expression in the adenoma tissue from patient A (lane 3) was considerably greater than the adjacent liver and similar to that of control liver. (B) We reprobed the same Northern blot for β-actin to control for RNA loading. These results are representative of 3 experiments without significant variation between experiments.