American Society of Hematology

Fig. 3.

$Fig. 3. Apoptosis-related proteins during drug-induced cell death. / (A) Whole-cell lysates were obtained from 2 × 106 cells from a representative CLL patient incubated in the absence (C) or presence of the FCM combination for 24 hours, and analyzed by Western blot. (B) Cytosolic and mitochondrial fractions were obtained from 50 × 106 cells incubated in the absence (C) or presence of the FCM combination for 24 hours. Mitochondrial fractions were either untreated or treated with alkali. All fractions were analyzed by Western blot. The position of the proteolytic fragment (85 kd) of PARP is indicated by an arrow.$

Apoptosis-related proteins during drug-induced cell death.

(A) Whole-cell lysates were obtained from 2 × 10⁶ cells from a representative CLL patient incubated in the absence (C) or presence of the FCM combination for 24 hours, and analyzed by Western blot. (B) Cytosolic and mitochondrial fractions were obtained from 50 × 10⁶ cells incubated in the absence (C) or presence of the FCM combination for 24 hours. Mitochondrial fractions were either untreated or treated with alkali. All fractions were analyzed by Western blot. The position of the proteolytic fragment (85 kd) of PARP is indicated by an arrow.

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