American Society of Hematology

Fig. 5.

$Fig. 5. Analysis of cytochrome c in drug-induced apoptosis. / (A) Flow cytometric analysis of cytochrome c staining. Cells from a representative CLL patient were incubated in the presence or absence of 5 μg/mL fludarabine, 10 μM dexamethasone, or the FCM combination for 24 hours, stained with anti–cytochrome c, and analyzed by flow cytometry. The percentage of cytochrome c–negative cells is indicated in each histogram. (B) Western blot of cytochrome c in mitochondrial and cytosolic fractions obtained from 50 × 106 cells of a representative CLL patient incubated in the presence or absence of FCM for 24 hours.$

Analysis of cytochrome c in drug-induced apoptosis.

(A) Flow cytometric analysis of cytochrome c staining. Cells from a representative CLL patient were incubated in the presence or absence of 5 μg/mL fludarabine, 10 μM dexamethasone, or the FCM combination for 24 hours, stained with anti–cytochrome c, and analyzed by flow cytometry. The percentage of cytochrome c–negative cells is indicated in each histogram. (B) Western blot of cytochrome c in mitochondrial and cytosolic fractions obtained from 50 × 10⁶ cells of a representative CLL patient incubated in the presence or absence of FCM for 24 hours.

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