Fig. 2.
Western blot of transiently expressed ADAMTS13.
Lysates and conditioned media from transfected HEK 293 cells transiently expressing ADAMTS13 were loaded on SDS-polyacrylamide gels, blotted to nitrocellulose, and visualized by murine monoclonal antibody 242/H1 directed against the catalytic domain of VWF-cp. The amount of lysate and conditioned medium loaded onto the gel was equivalent to approximately 1 × 106 cells. Lanes 1 and 3, conditioned medium and lysate from HEK 293 cells transfected with control vector pcDNA3.1; lanes 2 and 4, conditioned medium and lysate from cells transfected with VWF-cp expression vector pCMV/VWF-cp. M indicates protein standard in kDa.
