Cleavage of VWF subunits by rADAMTS13.

Western blot analysis of VWF fragments after VWF-cp digestion. VWF cleavage fragments were separated by SDS-PAGE under reducing conditions. VWF substrate (lane 1) was incubated with NHP (lane 2), buffer (lane 3), lysates, and conditioned media from HEK 293 transfections with the control vector (lanes 4 and 6) and with the VWF-cp expression plasmid (lanes 5 and 7). Lysates, conditioned media, and NHP were diluted 1:10 in the VWF-cp assay. M indicates protein standard in kilodaltons.