FL stimulation slows the differentiation of 32D cells expressing wild-type FLT3.
32D/FLT3 cells were washed and transferred from medium containing IL-3 to medium containing G-CSF (20 ng/mL) with and without FL (100 ng/mL) and cultured for 12 days. Fresh medium with G-CSF with and without FL was used to replace the old medium every 48 hours. (A) Cytospins were prepared on days 0, 9, and 12. The morphologic features of the cells were visualized by means of Wright-Giemsa staining followed by light microscopy. Original magnification, × 100. (B) Total cellular RNA was prepared from 1 × 107 32D/FLT3 cells after 0, 1, 3, 5, 7, and 9 days in medium containing G-CSF without FL (lanes 1-6) or with FL (100 ng/mL; lanes 7-12). RNA (15 μg) was then subjected to Northern blotting with MPO, lysozyme, and actin cDNA probes.
