American Society of Hematology

Fig. 2.

$Fig. 2. Most Lin− NK-cell progenitors are most abundant in the c-kitLo fraction of bone marrow cells. / Bone marrow cells bearing markers associated with various blood cell lineages were rigorously depleted with magnetic beads and high-speed cell sorting (as described in “Materials and methods”). The remaining Lin− cells were sorted into 3 fractions according to density of c-kit and cultured with SCF, FL, IL-7, and IL-15 for 7 days. Recovered cells were then counted and analyzed by flow cytometry for expression of B (CD19), NK (DX5), or myeloid (CD11b/Mac-1, not shown) lineage antigens (top panels). Absolute numbers of recovered cells of each type were then divided by the numbers of precursors used to initiate the cultures to obtain the yield per input values shown in the lower graph.$

Most Lin⁻ NK-cell progenitors are most abundant in the c-kit^Lo fraction of bone marrow cells.

Bone marrow cells bearing markers associated with various blood cell lineages were rigorously depleted with magnetic beads and high-speed cell sorting (as described in “Materials and methods”). The remaining Lin⁻ cells were sorted into 3 fractions according to density of c-kit and cultured with SCF, FL, IL-7, and IL-15 for 7 days. Recovered cells were then counted and analyzed by flow cytometry for expression of B (CD19), NK (DX5), or myeloid (CD11b/Mac-1, not shown) lineage antigens (top panels). Absolute numbers of recovered cells of each type were then divided by the numbers of precursors used to initiate the cultures to obtain the yield per input values shown in the lower graph.

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