American Society of Hematology

Fig. 4.

$Fig. 4. Most NK-cell precursors in the Lin−c-kitLo fraction are not directly sensitive to estrogen. / Lin− c-kitLo bone marrow cells were put into serum-free, stromal cell–free cultures along with vehicle (control) or estrogen (β-estradiol). One week later, the cultures were harvested and examined by flow cytometry for the production of B-lineage (CD19+) or NK-lineage (DX5+) lymphocytes. (A) Typical flow cytometry profiles are shown from one representative experiment. (B) Data from 9 independent experiments were normalized and expressed as average yields of B- and NK-lineage lymphocytes in estrogen-treated cultures relative to the untreated controls ± SE$

Most NK-cell precursors in the Lin⁻c-kit^Lo fraction are not directly sensitive to estrogen.

Lin⁻ c-kit^Lo bone marrow cells were put into serum-free, stromal cell–free cultures along with vehicle (control) or estrogen (β-estradiol). One week later, the cultures were harvested and examined by flow cytometry for the production of B-lineage (CD19⁺) or NK-lineage (DX5⁺) lymphocytes. (A) Typical flow cytometry profiles are shown from one representative experiment. (B) Data from 9 independent experiments were normalized and expressed as average yields of B- and NK-lineage lymphocytes in estrogen-treated cultures relative to the untreated controls ± SE

This Feature Is Available To Subscribers Only