Expression of Toll-like receptors and CD14 on neutrophils and monocytes.

Expression of TLR2, TLR4, and CD14 on peripheral blood neutrophils and monocytes was measured by fluorescence analysis of cells stained with anti-TLR2, anti-TLR4, anti-CD14, or isotype-control mAbs followed by PE-labeled goat anti–mouse Ig Ab. Isotype-control Ab–stained cells are shown as gray histograms. Specific mAb–stained cells are shown as black line histograms. Geometric mean fluorescence intensity (SD). Neutrophils: isotype control, 4.7 (0.3); TLR2, 22.6 (0.5); TLR4, 6.3 (0.3); and CD14, 42.1 (0.5). Monocytes: isotype control, 6.3 (0.3); TLR2, 96.6 (0.3); TLR4, 23.4 (0.2); and CD14, 1183 (0.3).