Kinetics of GM-CSF–induced TLR2 and CD14 expression.

Neutrophils were incubated with GM-CSF (100 U/mL) or medium alone for up to 3 hours at 37°C. At the indicated times, cells were stained and analyzed for expression of TLR2, CD14, and Class I MHC. Data are expressed as geometric mean fluorescence intensity of cells stained at t = 0 (immediately after isolation) or following incubation for 1, 2, or 3 hours prior to staining. Significance of difference between treated cells and untreated controls: **P < .001 by Probability Binning ChiT analysis (ChiT(X) > 330);P < .001 by Kolmogorov-Smirnoff analysis.