LPS enhances TLR2 and CD14 but not TLR4 expression on neutrophils.

Neutrophils and monocytes were incubated with LPS or medium alone for 2 hours prior to staining and FACS analysis. Isotype-control Ab–stained cells are shown as gray histograms. Specific mAb–stained cells are shown as black line histograms. Geometric mean fluorescence intensity (SD). Neutrophils treated with medium alone: isotype control, 7.9 (0.4); TLR2, 12.3 (0.3); TLR4, 8.5 (0.3); and CD14, 17.5 (0.3). Neutrophils treated with LPS: isotype control, 7.9 (0.4); TLR2, 18.2 (0.4); TLR4, 8.4 (0.3); and CD14, 27.1 (0.5). Monocytes treated with medium alone: isotype control, 12.9 (0.4); TLR2, 42.3 (0.5); TLR4, 23.5 (0.6); and CD14, 633 (1.6). Monocytes treated with LPS: isotype control, 12.9 (0.4); TLR2, 48.6 (0.6); TLR4, 14.8 (0.5); CD14, 566 (1.8). Significance of difference between treated cells and untreated controls: **P < .001 by Probability Binning ChiT analysis (ChiT(X) > 500); P < .001 by Kolmogorov-Smirnoff analysis.