![Fig. 4. Effects of stressed apoptotic 12B1-D1 cells on DCs. / (A) Stressed apoptotic 12B1-D1 cells induce IL-12 production by DCs. ELISPOT assays were performed to measure the IL-12 secretion by DCs. A total of 3 × 105 DCs were cocultured with media with heat-stressed or with nonstressed apoptotic 12B1-D1 cells at a 1:1 ratio for 24 hours in the presence of 10 ng/mL GM-CSF and IL-4 for 24 hours. (B) Stressed apoptotic 12B1-D1 cells increase DC capacity to stimulate allogeneic splenocyte proliferation in MLR. DCs were cocultured with heat-stressed or nonstressed apoptotic 12B1-D1 cells at a 1:1 ratio for 24 hours in the presence of 10 ng/mL GM-CSF and IL-4 for 24 hours. DCs were then collected and treated with mitomycin C and washed as described in “Materials and methods.” A total of 105 splenocytes from C57BL6 mice were added per well and cultured with the indicated ratio of pretreated BALB/c DCs for 4 days. [3H]thymidine was added, and the cells were cultured for an additional 18 hours before the incorporated radioactivity was counted. Representative data from 1 of 3 experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/12/10.1182_blood-2002-05-1389/5/h82323488004.jpeg?Expires=1735538074&Signature=XjMW630WFhuFmr1EIuoLRlUxVW989AH0sH-3SnEl38fSvmQoPuDiX1RpOPmHocb8dcbybttuhiQAty-nEUwoHkTUJ8r2MZmZT5PfpomgbOo9EbEH6nGtkn8HLA3hZMLS4gr2YWW0eE80~HdtZ5FxSHnEOY6kS58y42jLegsHHyPC0J2tHj7bS0RFcy6ht1LFmlv00Wopj9jXKsoUf15~LAhQM5b9xZUECVAJw~8tKGQcqKtAv6-xDcB6QcMzl3wCWgpLqZrjpfGxA9qQE0UCRZbz~iWPQIBMiWG3z2x24xIa00gtz~oSAKgVAQF6gFTJwFbJVUuXHUGv9v8J9cQ~AA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of stressed apoptotic 12B1-D1 cells on DCs.
(A) Stressed apoptotic 12B1-D1 cells induce IL-12 production by DCs. ELISPOT assays were performed to measure the IL-12 secretion by DCs. A total of 3 × 105 DCs were cocultured with media with heat-stressed or with nonstressed apoptotic 12B1-D1 cells at a 1:1 ratio for 24 hours in the presence of 10 ng/mL GM-CSF and IL-4 for 24 hours. (B) Stressed apoptotic 12B1-D1 cells increase DC capacity to stimulate allogeneic splenocyte proliferation in MLR. DCs were cocultured with heat-stressed or nonstressed apoptotic 12B1-D1 cells at a 1:1 ratio for 24 hours in the presence of 10 ng/mL GM-CSF and IL-4 for 24 hours. DCs were then collected and treated with mitomycin C and washed as described in “Materials and methods.” A total of 105 splenocytes from C57BL6 mice were added per well and cultured with the indicated ratio of pretreated BALB/c DCs for 4 days. [3H]thymidine was added, and the cells were cultured for an additional 18 hours before the incorporated radioactivity was counted. Representative data from 1 of 3 experiments are shown.
