Fig. 5.
![Fig. 5. Immunization of mice with stressed apoptotic 12B1-D1 cells induces IFN-γ, IL-2 secretion by splenocytes and T-cell proliferation. / Heat-stressed or nonstressed 12B1-D1 cells (2 × 106) were treated with 40 nM AP20187 for 6 hours and then injected to BALB/c mice subcutaneously. Splenocytes from immunized mice were harvested 5 days later and restimulated with mitomycin C–treated 12B1-D1 cells. (A) CTLL-2 bioassay was used to determine the IL-2 production. (B) IFN-γ secretion was determined by ELISPOT. (C) T-cell proliferation was determined by [3H]thymidine incorporation. Representative data from 1 of 3 experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/12/10.1182_blood-2002-05-1389/5/h82323488005.jpeg?Expires=1735536354&Signature=KpSCwJJ-1aRWzWtJkJQQsOetVgZGTxbeSFg3r2UpdcRXLmyi8KbU6yEekWDi3sgpUOLYrqKQI0Ol0IuAxIxe5-0zg1UD6jvvFQnAadRY5kW9fnj~RtN4oO0LaLkPFiB3XUuXB5cRTWlceRmrQqmW7tMQ0aTFr~r5fr2FMxAXTK5ieo9MJ5dnTDysVD15BgMoBARv9KpqGPxBzbRzdDfOJ~VMNgNGif4AwGs1lWyBw8vZ4we0jj2Ga2tcbMGtfQ-fA2dsM6FOIYFHX54-EYxRYyb0LnSyqdQfHpuQ1fNKHgx1s5VZCfrVbo-Nbqu9~58luLD20Hs1At2VTMTGwgOwOg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunization of mice with stressed apoptotic 12B1-D1 cells induces IFN-γ, IL-2 secretion by splenocytes and T-cell proliferation.
Heat-stressed or nonstressed 12B1-D1 cells (2 × 106) were treated with 40 nM AP20187 for 6 hours and then injected to BALB/c mice subcutaneously. Splenocytes from immunized mice were harvested 5 days later and restimulated with mitomycin C–treated 12B1-D1 cells. (A) CTLL-2 bioassay was used to determine the IL-2 production. (B) IFN-γ secretion was determined by ELISPOT. (C) T-cell proliferation was determined by [3H]thymidine incorporation. Representative data from 1 of 3 experiments are shown.
