Immunization of mice with stressed apoptotic 12B1-D1 cells induces tumor-specific CTLs.

BALB/c were immunized with saline heat-stressed or nonstressed 12B1-D1 cells that had been pretreated with 40 nM AP20187 for 6 hours. Splenocytes from immunized mice were harvested 5 days later and restimulated with mitomycin C–treated 12B1-D1 cells for 5 days. Stimulated effector cells were tested for cytolytic activity against (A) 12B1-D1 cells, (B) parental 12B1 cells, or (C) A20 cells, using a 4- to 6-hour cytotoxicity assay. Representative data from 1 of 3 experiments are shown.