Down-regulation of CD43 mRNA during K562 activation.

Total RNA was prepared from K562 cells treated with PMA for the hours indicated. RNA was then subjected to Northern blot analysis using anEcoRI/NcoI fragment (CEM-E/N) isolated from theCD43 cDNA clone pCEM1.7, which has previously been used to detect CD43 mRNA.11 As a control for RNA loading, the same Northern blot hybridized with the CD43-specific probe was subsequently hybridized with a probe that specifically recognizes GAPDH mRNA.53 The position of migration of 28S ribosomal RNA is marked.