Fig. 2.
Fig. 2. Repression of the CD43 promoter during K562 activation. / The construct p43Wt containing nucleotides −2 to +99 of theCD43 gene promoter was transfected into K562 cells along with the control plasmid pRSV-β encoding β-galactosidase. Transfected cells were then either left untreated (−PMA) or treated for 12 hours with PMA (+PMA) prior to harvesting. Luciferase values were measured and normalized against β-galactosidase levels to correct for transfection efficiency. Expressed as histograms are the levels of luciferase activity directed by p43Wt in untreated and PMA-treated K562 cells after division by the background activity conferred by the control plasmid pATLuc. Each histogram represents the mean ± SD of 3 independent transfection experiments.

Repression of the CD43 promoter during K562 activation.

The construct p43Wt containing nucleotides −2 to +99 of theCD43 gene promoter was transfected into K562 cells along with the control plasmid pRSV-β encoding β-galactosidase. Transfected cells were then either left untreated (−PMA) or treated for 12 hours with PMA (+PMA) prior to harvesting. Luciferase values were measured and normalized against β-galactosidase levels to correct for transfection efficiency. Expressed as histograms are the levels of luciferase activity directed by p43Wt in untreated and PMA-treated K562 cells after division by the background activity conferred by the control plasmid pATLuc. Each histogram represents the mean ± SD of 3 independent transfection experiments.

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