Fig. 1.
Regulation of LTα and LTβ transcription in primary human cells.
(A) Northern blot analyses of LTα and LTβ mRNA levels in human PBMC. (B) Location of LTα and LTβ hybridization probes within the human TNF-α locus. Cells were treated with 20 ng/mL PMA plus 2 μM ionomycin (P/I) in presence (+) or absence (−) of CsA (400 nM), as indicated. CsA was added 30 minutes prior to activation. No act indicates nonactivated control. RNA (10μg) from 5 to 10 × 106 cells taken at indicated time points was used for Northern analysis. Ethidium bromide staining of 18S RNA at the bottom panel is shown as RNA loading control.
