Fig. 3.
CsA inhibits LTα protein expression in PBMCs.
Cells were treated with 20 ng/mL PMA plus 2 μM ionomycin (P/I) in the presence (+) or absence (−) of 400 nM CsA for the indicated time in hours (h). CsA was added 30 minutes prior to activation. For each time point extracts from 108 cells were immunoprecipitated with antipeptide rabbit polyclonal serum no. 3236. Proteins were detected by subsequent Western blotting with the same antiserum. Extracts from CHO cells stably transfected with LTα and LTβ expression construct were used as positive control; untransfected CHO cells were used as negative control.
