Fig. 7.
Fig. 7. Bcl-XL and Bax protein expression in DP16.1/p53ts cells. / (A) DP16.1/p53ts cells were cultured at 32°C in the presence or absence of EPO for the times indicated. Protein extracts were fractionated by SDS-PAGE and immunoblotted separately with antibodies against Bcl-XL and Bax. Proteins were visualized by enhanced chemiluminescence. (B) DP16.1/p53ts cells were cultured at 32°C in the presence or absence of EPO for 24 hours. Cell extracts were prepared and analyzed by 2-dimensional gel electrophoresis followed by Western blot analysis using antibodies against Bcl-XL. A portion of the extract prepared from the EPO-stimulated cells was incubated with λ-phosphatase (20 U/μL) prior to analysis. The arrow in the top panel points to the more slowly migrating isoform of Bcl-XL that is lost on EPO stimulation.

Bcl-XL and Bax protein expression in DP16.1/p53ts cells.

(A) DP16.1/p53ts cells were cultured at 32°C in the presence or absence of EPO for the times indicated. Protein extracts were fractionated by SDS-PAGE and immunoblotted separately with antibodies against Bcl-XL and Bax. Proteins were visualized by enhanced chemiluminescence. (B) DP16.1/p53ts cells were cultured at 32°C in the presence or absence of EPO for 24 hours. Cell extracts were prepared and analyzed by 2-dimensional gel electrophoresis followed by Western blot analysis using antibodies against Bcl-XL. A portion of the extract prepared from the EPO-stimulated cells was incubated with λ-phosphatase (20 U/μL) prior to analysis. The arrow in the top panel points to the more slowly migrating isoform of Bcl-XL that is lost on EPO stimulation.

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