K25V administered subcutaneously without adjuvant is immunogenic in Tg mice.

K25V was dissolved at 5 mM in 90% N-saline/10% DMSO and diluted in N-saline to deliver the amount of peptide shown on the x-axis. Tg HLA A2.1/K^b [n = 6 (150 nmol), 14 (100 nmol), 8 (50 nmol), and 2 (10 and 25 nmol)] mice were immunized once subcutaneously at the base of the tail with peptide and no additional adjuvant. After 2 weeks, spleens were harvested, and IVS were done as described in “Materials and methods.” Targets were T2 cells loaded with specific (pp65_495-503, filled symbols) and nonspecific (p53_149-157, open symbols) peptides as described in “Materials and methods.” Means and SE were calculated at each effector-target (E/T) ratio for all evaluated mice, and significantP values are indicated.