Fusion peptides with CpG ss-ODN administered intranasally stimulate potent systemic immunity.
(A) Fusion peptide K25V, either 25 (●) or 50 nmol (■) mixed with 25 μg CpG ss-ODN or 100 nmol without ss-ODN (▴) was administered into the nares, as described in “Materials and methods.” Under anesthesia, 15 μL was introduced into each naris for a total of 30 μL total/mouse. (B) The same as panel A, except a booster of the identical amount of peptide and DNA as described for panel A was given 2 weeks after the first intranasal immunization. HLA A2.1/Kb mice were immunized for 2 (single) or 3 (booster) weeks, spleens removed, and 1 IVS was performed for 7 days. Afterward, a CRA was conducted as described in the legend to Figure 1 and in “Materials and methods.”
