Formation of ULVWF strings on different types of endothelial cells.

Washed normal platelets were perfused over histamine-stimulated endothelial cells from human umbilical veins (HUVECs) and arteries (HUAECs), as well as from coronary arteries (HCAECs) and lung microvasculature (HMVECs), under a shear stress of 2.5 dyne/cm². Formation of ULVWF strings with adherent platelets was evaluated after 2 minutes of perfusion and quantified in 20 continuous view fields (× 400). The results are expressed as means ± SEM, * Student t test, n = 7,P < .05.