Fig. 7.
Cleavage of the ULVWF strings with adherent platelets by normal plasma, TTP plasma, or ADAMTS-13.
Washed normal platelets were perfused over histamine-stimulated HUVECs at 2.5 dyne/cm2 shear stress for 2 minutes to allow formation of ULVWF strings with adherent platelets. Then, either Tyrode buffer, normal plasma, TTP plasma, or partially purified VWF-cleaving metalloprotease was perfused for an additional 2 minutes (for normal plasma and purified VWF-cleaving metalloprotease) or 10 minutes (for buffer or TTP plasma). The number of ULVWF strings with adherent platelets was quantified in 20 continuous view fields (× 400), and the results are expressed as means ± SEM.
