VE-cadherin distribution on cultured human umbilical vein endothelial cells (HUVECs) in the presence of PMN transmigration.

(A-B) Specimens were fixed with 4% paraformaldehyde in the presence of 0.5% Triton-X 100 and subsequently immunostained with anti–VE-cadherin antibody and fluorescence-conjugated secondary antibody. (C-F) Specimens were prelabeled with the primary antibody against VE-cadherin, fixed, and finally labeled with the secondary antibody. Confocal fluorescence micrographs B, D, and F were taken from the same specimens from which phase-contrast micrographs A, C, and E were taken. Triangle symbols indicate the locations of transmigrating PMNs. PMNs on top of the endothelial cells are marked by arrows, and PMNs already transmigrated are marked by asterisks.