Transendothelial migration of PMNs on human umbilical vein tissue.

(A-E) Living confocal images with PMNs stained with FM 1-43 (red) and endothelial VE-cadherin immunostained with Alexa Fluor 488 (green), whereas panel F shows a scanning electron micrograph of the same specimen after fixation. (A-C) Individual optical sections obtained from different depths; (D) a depth-combined image of FM 1-43; (E) a depth-combined image of both FM 1-43 and Alexa Fluor 488. Among numerous PMNs shown here, 2 transmigrated PMNs are indicated by asterisks, and 3 adhered PMNs are indicated by triangles.