In vivo experimental system to test resistance of GPI anchor–defective hematopoietic cells to cytotoxic cells.
Pigaflox males were crossed withhCMV-Cre transgenic females. Female embryos that had theCre transgene became heterozygous for the Pigadisruption and mosaic for Piga expression due to random X inactivation. Fetal liver cells of the mosaic embryos on 14 dac were transferred to lethally irradiated B6 recipients together with lineage marker–negative bone marrow cells of bm12 mice, with or without purified CD4+ T cells from bm12 mice. To distinguish fetal liver–derived cells from recipient-derived and bm12 bone marrow–derived cells, mice with the Ly5.1 allele were used as a source of fetal livers and mice with the Ly5.2 allele were used for the recipients and as a source of bone marrow.
