Detection of CDR3 sequences before and after ATG-based treatment.

The TCR-Vβ 18 family of UPN 8 contained a clonal T-cell expansion that persisted 6 months after ATG treatment. The detection of the target DNA sequence was performed on TCR-Vβ 18 PCR product using an oligonucleotide probe with the shared CDR3 sequence of the clonal T-cell expansion. Lane 1 was loaded with 1 ng and lane 2 with 0.1 ng target DNA product from pretreatment PBMCs of UPN 8. Lane 3 was loaded with 1 ng and lane 4 with 0.1 ng target DNA product from posttreatment PBMCs of UPN 8. Lane 5 is a negative control in which 2 ng of the reverse-transcribed PCR product of the TCR-Vβ 18 family from a healthy donor was probed.