American Society of Hematology

Fig. 5.

$Fig. 5. Association of acute graft versus host disease and steroid therapy with decreased functional fractions of CMV-specific CD8+ T cells. / (A) Functional fractions of CMV-specific CD8+ T cells were assessed using a combination of HLA-pp65 tetramer staining and cytokine flow cytometry as previously discussed. Individuals who experienced acute GVHD (○, median 25%) also had a lower functional fraction of CMV-specific CD8+ T cells (●, median 49% for those with no acute GVHD) (P = .05). (B) Dysfunction of CMV-specific CD8+ T cells in subjects treated with steroids. Individuals receiving tacrolimus alone at the time of sampling (●, median 48%) had a higher fraction of functional CMV-specific CD8+ T cells than those subjects receiving steroids in addition to tacrolimus (○, median 27%) (P = .03). All P values attained by the Mann-Whitney test.$

Association of acute graft versus host disease and steroid therapy with decreased functional fractions of CMV-specific CD8⁺ T cells.

(A) Functional fractions of CMV-specific CD8⁺ T cells were assessed using a combination of HLA-pp65 tetramer staining and cytokine flow cytometry as previously discussed. Individuals who experienced acute GVHD (○, median 25%) also had a lower functional fraction of CMV-specific CD8⁺ T cells (●, median 49% for those with no acute GVHD) (P = .05). (B) Dysfunction of CMV-specific CD8⁺ T cells in subjects treated with steroids. Individuals receiving tacrolimus alone at the time of sampling (●, median 48%) had a higher fraction of functional CMV-specific CD8⁺ T cells than those subjects receiving steroids in addition to tacrolimus (○, median 27%) (P = .03). All P values attained by the Mann-Whitney test.

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