Diagnostic pathway for AIHA. DAggT, direct agglutination test; DIIHA, drug-induced immune hemolytic anemia; HA, hemolytic anemia; HDN, hemolytic disease of the newborn; HTR, hemolytic transfusion reaction; PLS, passenger lymphocyte syndrome; RT, room temperature. *The final diagnosis of CHAD or mixed AIHA is based on the overall clinical picture, including supportive serological findings. ^†For example, the thermal amplitude. **Saline-suspended red cells and patient’s serum at room temperature for 30 to 60 minutes. Adapted from Hill et al with permission.¹²