Antagonism of the proapoptotic effect of LY294002 by the pan-caspase inhibitor zvad.fmk and the caspase-3 inhibitor z-devd-fmk.

B-CLL cells were incubated in medium alone or with 10 μM LY294002. Cells were preincubated either with the broad-spectrum caspase inhibitor zvad.fmk (100 μM) or the caspase-3 inhibitor z-devd-fmk (200 μM) 30 minutes prior to adding LY294002 to the culture medium. Apoptosis was detected by annexin/PI staining after 24 hours. One representative experiment of 3 is shown.