Activity of the lymphotropic TFs.

Activities of AP-1, NF-κB, and NFAT in nuclear extracts isolated from PBLs of 2 ITP patients in acute phase and in remission and a healthy pediatric control are shown. EMSAs were performed with labeled probes, as described in “Patients, materials, and methods,” and protein extracts from PBLs of the patients and the healthy control before or after mitogenic stimulation of the cells with PMA and ionomycine. Lane 1, free probes; lane 2, healthy control, ex vivo cells; lane 3, healthy control, stimulated cells; lane 4, first patient in acute phase, ex vivo cells; lane 5, first patient in acute phase, stimulated cells; lane 6, second patient in acute phase, ex vivo cells; lane 7: second patient in acute phase, stimulated cells; lane 8, first patient in remission, ex vivo cells; lane 9, first patient in remission, stimulated cells; lane 10, second patient in remission, ex vivo cells; lane 11, second patient in remission, stimulated cells. Arrows show active TF complexes. NFAT-A indicates NFAT-binding activator; NFAT-S, NFAT-binding silencer.