American Society of Hematology

Fig. 3.

$Fig. 3. Relative amounts of hyaluronan in medium from healthy donor and multiple myeloma patient–derived bmMPC cultures. / FACE analysis of HA in the medium ethanol precipitate fractions of bmMPC cultures derived from a healthy donor (lanes 1-4) and a multiple myeloma patient (lanes 5-8). Cultures were incubated as indicated in the presence (+; lanes 3, 4, 7, and 8) or absence (−; lanes 1, 2, 5, and 6) of dexamethasone (DEX) as described in “Materials and methods.” Samples were digested as indicated with (+; lanes 2, 4, 6, and 8) or without (−; lanes 1, 3, 5, and 7) hyaluronidase SD, chondroitinase ABC, and glucoamylase (EZ) as described in “Materials and methods.” The gel image was exposed such that all pixels were within the linear 12-bit depth range required for quantitation. The percent HA detected as AMAC-derivatized ΔDiHA in each culture is listed above each lane and is normalized to the (−) DEX multiple myeloma value in each image. Note the absence of AMAC-derivatized ΔDiHA or ΔDi0S in the absence of enzyme digestion and the similar intensity of the AMAC-derivatized ΔDi0S (the unsulfated disaccharide of chondroitin sulfate) in each sample. Each lane represents 0.5% (1:200) of the total medium sample. Data presented are representative of 4 healthy donors and 4 myeloma patients. Similar results were observed in all donors.$

Relative amounts of hyaluronan in medium from healthy donor and multiple myeloma patient–derived bmMPC cultures.

FACE analysis of HA in the medium ethanol precipitate fractions of bmMPC cultures derived from a healthy donor (lanes 1-4) and a multiple myeloma patient (lanes 5-8). Cultures were incubated as indicated in the presence (+; lanes 3, 4, 7, and 8) or absence (−; lanes 1, 2, 5, and 6) of dexamethasone (DEX) as described in “Materials and methods.” Samples were digested as indicated with (+; lanes 2, 4, 6, and 8) or without (−; lanes 1, 3, 5, and 7) hyaluronidase SD, chondroitinase ABC, and glucoamylase (EZ) as described in “Materials and methods.” The gel image was exposed such that all pixels were within the linear 12-bit depth range required for quantitation. The percent HA detected as AMAC-derivatized ΔDiHA in each culture is listed above each lane and is normalized to the (−) DEX multiple myeloma value in each image. Note the absence of AMAC-derivatized ΔDiHA or ΔDi0S in the absence of enzyme digestion and the similar intensity of the AMAC-derivatized ΔDi0S (the unsulfated disaccharide of chondroitin sulfate) in each sample. Each lane represents 0.5% (1:200) of the total medium sample. Data presented are representative of 4 healthy donors and 4 myeloma patients. Similar results were observed in all donors.

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