Effect of C5aR antagonist on CR3 (CD11b) expression and effect of anti-CR3 antibodies on the oxidative burst.
The C5aR antagonist inhibited E coli–induced CD11b expression dose dependently in contrast to the control peptide (left panel). ▵ indicates baseline value (T0); ▴, whole blood incubated 10 minutes with E coli(1 × 108 bacteria per milliliter); ●, C5aR antagonist; and ○, control peptide. F(ab′)2 fragments of anti-CR3 (anti-CD18 and anti-CD11b) inhibited granulocyte oxidative burst dose dependently, in contrast to a control F(ab′)2(right panel). ▵ indicates baseline value (T0); ▴, whole blood incubated 10 minutes with E coli(1 × 108 bacteria per milliliter); ■, anti-CD18; ▪, anti-CD11b; ●, C5aR antagonist (2 μM); and ○, control mAb. One representative of 5 experiments is shown.
