Fig. 10.
Effect of anti-CR3 and anti-FcγR on phagocytosis.
Anti-CR3 (CD11b) antibody dose dependently inhibited granulocyte (left) and monocyte (right) phagocytosis of E coli preopsonized with C3 and IgG, in contrast to anti-FcγRI (CD64), anti-FcγRII (CD32), and anti-FcγRIII (CD16) antibodies, which had no effect. All antibodies were F(ab′)2 fragments of mouse mAbs blocking the function of the receptors (see “Materials and methods”). One representative of 4 experiments is shown. ▵ represents baseline value (T0); ▴, whole blood incubated 20 minutes with E coli (1 × 108 bacteria per milliliter); ●, anti-CD11b; ○, anti-CD16; ■, anti-CD32; and ▪, anti-CD64.
