Immunolocalization of 4.1R and NuMA in erythroid and lymphoid cells.

Column A shows 4.1 isoform distribution; column B, NuMA distribution; column C, colocalization of various 4.1 isoforms and NuMA; column D, higher magnification of the mitotic cells shown in column C; column E, results obtained with the use of lymphoid cells. In all of these, only the high magnifications are shown. Bar indicates 5 μm. AbCT gave a positive reaction with 4.1R in the control and with CO.2 in the patient. AbCO recognized only the missense sequence of CO.1 in the patient (note the complete absence of fluorescence in the control, lane 3). Normal 4.1R (control), and CO.2 and CO.1 (homozygote) showed a punctate distribution within the cytoplasm and the nucleus of interphasic and dividing cells. The 4.1R partly concentrated at the center of each mitotic pole in dividing cells. AbNuMA gave a positive reaction in both the control and the homozygote. NuMA had a diffuse distribution in the nucleus in interphasic cells and concentrated almost entirely in the mitotic spindle poles in dividing cells. The 4.1R and CO.2, on the one hand, and AbNuMA, on the other, colocalized in control and homozygote mitotic cells, while CO.1 was missing from the spindle poles (AbCO plus AbNuMA; lane 4, columns C, D, and E).