Effect of Bay 11-7082 on the expression of cyclin D1, cyclin D2, and Bcl-xL in HTLV-I–infected T-cell lines.
(A) Total RNA (20 μg) was extracted from HTLV-I–infected T-cell lines following treatment without or with 5 μM Bay 11-7082 for 24 hours. Northern blot analysis was performed with cyclin D1, cyclin D2, and Bcl-xL cDNA probes. The GAPDH probe was used to demonstrate equivalent RNA levels in each sample. (B) Time course of cyclin D1, cyclin D2, and Bcl-xL mRNA expression in SLB-1 cells treated with Bay 11-7082. Total RNA (20 μg) was extracted from SLB-1 cells following treatment without or with Bay 11-7082 for the indicated times. (C) Expression of cyclin D1, cyclin D2, and Bcl-xL at the protein level in SLB-1 cells. SLB-1 cells were treated without or with Bay 11-7082 for 48 hours. After treatment, the expression of these proteins was examined by Western blot. (D) Graphic quantitation of relative levels of cyclin D1, cyclin D2, and Bcl-xL mRNA expression in cells. GAPDH bands were measured by densitometry and the values were used to normalize for densitometric quantitations of cyclin D1, cyclin D2, and Bcl-xL.
