Bay 11-7082 reduces cell growth and induces apoptosis of primary ATL cells.

ATL cells were cultured at 1 × 10⁶ cells/mL without or with 5 μM Bay 11-7082 for 48 hours. (A) Effect of Bay 11-7082 in ATL cells on the activation of NF-κB assessed by EMSA with NF-κB probe. (B) NF-κB subunit specificity was determined by using antibodies to the NF-κB components p50, p65, c-Rel, and p52, resulting in supershift. (C) ATL cell growth was assessed by the WST-1 method. (D) Induction of apoptosis of ATL cells following inhibition of NF-κB. Data represent the mean ± SD percentages of apoptotic cells from 3 independent experiments for both untreated cells (open bars) and cells treated with 5 μM Bay 11-7082 (solid bars).