Effect of phagocytic stimulation with opsonizedSaccharomyces cerevisiae (OSC) on Ca⁺⁺mobilization induced by IL-8, NAP-2, or C5a in human neutrophils.

(A-C) PMNs were treated with Fura-2 am and incubated alone (line 1) or treated with OSC (line 2) for 20 minutes, followed by the addition of IL-8 at 75 ng/mL (panel A), NAP-2 at 75 ng/mL (panel B), or C5a at 100 ng/mL (panel C). Levels of Ca⁺⁺ are presented as fluorescence ratio determined from background-corrected fluorescence exited alternately at 340 and 380 nm (F340/F380). Panels A, B, and C are based on data of one representative experiment. (D) Mean ± SD of percentages of Ca⁺⁺influx in response to IL-8 (n = 11), NAP-2 (n = 7), or C5a (n = 7) in phagocytosing neutrophils compared with control cells. The statistical differences between groups were determined by the Student t test, with P < .05 considered significant. *P < .05 versus untreated incubated control.