Genomic structure of the PRV1 gene.

The intron-exon structure of PRV1 was elucidated by sequence comparison between the cloned cDNA and sequences from BAC bc52850 and BAC 338531 obtained through the human genome project. Exons are depicted as boxes and introns as a line. Exons 3, 4, 7, and 8 contain the cysteine-rich domains, which define the uPAR/CD59/Ly6 family of cell surface receptors. Exons 4 through 9 are duplicated and form an adjacent inverted PRV1 pseudogene (labeled 9′ to 4′).SacI DNA restriction sites and the generated fragment lengths are illustrated.